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排序方式: 共有214条查询结果,搜索用时 31 毫秒
11.
Toyokazu KOBAYASHI Hiroshi KOIE Arisa WATANABE Arisa INO Kazuya WATABE Mujo KIM Kiichi KANAYAMA Kazuya OTSUJI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):503-506
We examined the effects of chicken egg hydrolysate (also known as “bone peptide” or BP)
on bone metabolism in 5- to 8-month-old orchidectomized dogs. The bone formation marker
serum bone alkaline phosphatase (BAP) and the bone resorption marker urine
deoxypyridinoline (DPD) were used as indicators to measure changes in bone metabolism. The
following results were observed that Serum BAP was higher in dogs fed BP-enriched food
throughout the clinical investigation. Serum BAP was statistically significantly higher in
dogs fed BP-enriched food than in dogs fed non-BP-enriched food at 2 months after
orchidectomy. This suggests that BP promoted bone formation immediately after
orchidectomy. 相似文献
12.
Saori MIURA Noriyuki HORIUCHI Kotaro MATSUMOTO Yoshiyasu KOBAYASHI Shin-ichiro KAWAZU Hisashi INOKUMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):883-887
Monoclonal integration of bovine leukemia virus (BLV) proviral DNA into bovine genomes
was detected in peripheral blood from two clinical cases of enzootic bovine leukosis (EBL)
without enlargement of superficial lymph nodes. A BLV-specific probe hybridized with 1 to
3 EcoRI and HindIII fragments in these 2 atypical EBL
cattle by Southern blotting and hybridization, as well as in 3 typical EBL cattle. The
probe also hybridized to a large number of EcoRI and
HindIII fragments in 5 cattle with persistent leukosis. These results
suggest that the detection of monoclonal integration of BLV provirus into the host genome
may serve as a marker of monoclonal proliferation and malignancy in difficult to diagnose
EBL cattle. 相似文献
13.
14.
Satoshi KAMBAYASHI Masaya IGASE Kosuke KOBAYASHI Ayana KIMURA Takako SHIMOKAWA MIYAMA Kenji BABA Shunsuke NOGUCHI Takuya MIZUNO Masaru OKUDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1405-1412
Hypoxic conditions in various cancers are believed to relate with their
malignancy, and hypoxia inducible factor-1α (HIF-1α) has been shown to be a major
regulator of the response to low oxygen. In this study, we examined HIF-1α expression in
canine lymphoma using cell lines and clinical samples and found that these cells expressed
HIF-1α. Moreover, the HIF-1α inhibitors, echinomycin, YC-1 and 2-methoxyestradiol,
suppressed the proliferation of canine lymphoma cell lines. In a xenograft model using
NOD/scid mice, echinomycin treatment resulted in a dose-dependent regression of the tumor.
Our results suggest that HIF-1α contributes to the proliferation and/or survival of canine
lymphoma cells. Therefore, HIF-1α inhibitors may be potential agents to treat canine
lymphoma. 相似文献
15.
Katsuki TANAKA Hikaru ADACHI Hironobu AKASAKA Junya TAMAOKI Yuji FUSE Makoto KOBAYASHI Takio KITAZAWA Hiroki TERAOKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1050
We reported the involvement of oxidative stress and prostaglandins including thromboxane and prostacyclin in pre-cardiac edema (early edema) caused by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). While the involvement of oxidative stress in TCDD-induced toxicity has been frequently reported, the mechanism of its action is still unclear. In the present study, oxidative stress inducers including paraquat, hydrogen peroxide (H2O2) and rotenone augmented early edema (edema) induced by a low concentration of TCDD (0.1 ppb) at 55 hr post fertilization (hpf), while each of them alone did not cause edema. Edema caused by TCDD plus oxidative stress inducers was almost abolished by antioxidants, an antagonist for thromboxane receptor (ICI-192,605) and an agonist for prostacyclin receptor (beraprost), suggesting that the site of action of these inducers was in the regular signaling pathway after activation of aryl hydrocarbon receptor type 2 (AHR2) by TCDD. Oxidative stress inducers also enhanced edema caused by an agonist for the thromboxane receptor (U46619), and the enhancement was also inhibited by antioxidants. Sulforaphane and auranofin, activators of Nrf2 that is a master regulator of anti-oxidative response, did not affect U46619-evoked edema but almost abolished TCDD-induced edema and potentiation by paraquat in both TCDD- and U46619-induced edema. Taken together, the results suggest that oxidative stress augments pre-cardiac edema caused by TCDD via activation of thromboxane receptor-mediated signaling in developing zebrafish. As paraquat and other oxidative stress inducers used also are environmental pollutants, interaction between dioxin-like compounds and exogenous source of oxidative stress should also be considered. 相似文献
16.
Abstract. The pathogenicity of Renibacterium salmoninarum to carp, Cyprinus carpio L., and rainbow trout, Salmo gairdneri Richardson, was investigated. All carp injected with 4·8 × 108 cells/fish, or 4·8 × 107 cells/fish survived for 38 days. R. salmoninarum was isolated from all moribund fish, but not from the kidney of surviving fish, although R. salmoninarum antigen was detected in several of these fish by the dot blot assay. On the other hand, mortality in rainbow trout was 95% in the fish injected with 4·8 × 108 cells/fish, and 15% in those which received 4·8 × 107 cells/fish. R. salmoninarum antigen was detected by the dot blot assay in all surviving rainbow trout. The number of R. salmoninarum cells was immediately decreased by carp or rainbow trout serum, and the serum bactericidal activity of carp was higher than that of rainbow trout. Carp blood leucocytes had higher phagocytic activity than those of rainbow trout. 相似文献
17.
18.
Yuki YAMAMOTO Yoshihiko KOBAYASHI Kiyoshi OKUDA 《The Journal of reproduction and development》2014,60(1):73-77
Isolated stromal cells from the ampullary and isthmic parts of bovine oviductal tissues
were cultured in monolayer and spheroid (cell aggregate) systems. Prostaglandin F2α (PGF)
plays a crucial role in oviductal contraction and is produced by oviductal epithelial
cells in cattle. Since stromal cells of many organs produce PGF, PGF production by bovine
oviductal stromal cells was investigated. After PGF synthesis was confirmed, the utility
of isolation and culture methods for oviductal stromal cells was evaluated by PGF
production in the present study. The homogeneity of the cells was > 99%. PGF production
of the cells was increased by tumor necrosis factor-α. The stromal cells aggregated and
formed a spheroid by the treatments with several reagents. PGF production was higher in
the spheroid culture than in the monolayer culture. The isolation and culture methods
described here will facilitate studies of the physiological function of bovine oviductal
stromal cells. 相似文献
19.